RNASET2 ELISA Kit

Produktnummer ABIN2044461

Dieses Human RNASET2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human RNASET2 zu quantifizieren.

Kurzübersicht für RNASET2 ELISA Kit (ABIN2044461)

Target: RNASET2 (Ribonuclease T2 (RNASET2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails RNASET2 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.069 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ribonuclease T2 (RNASET2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease T2 (RNASET2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease T2 (RNASET2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease T2 (RNASET2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease T2 (RNASET2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RNASET2

Target: RNASET2 (Ribonuclease T2 (RNASET2))